Collagen type II expression in the intervertebral disc after cigarette smoke exposure: An experimental study using wistar rats

Background: Low back pain is often caused by the degeneration of intervertebral disc. Cigarette smoking has been found to be related disturbance disc function. This study aimed evaluate type II collagen expression in after cigarette smoke exposure with an experimental using male wistar rats. Methods: research was post-test only group design conducted January April 2021. The samples were 21 adult rats which divided into 3 groups. There included control (without exposure), 9 Group I (4 weeks and (8 exposure). Immunohistochemical (IHC) staining microscopic observation done vertebral specimen expression. 2 observers whose results compared tested kappa reliability test. Data analysed chi square Results: a total this study. All from showed strong staining, while moderate dominant (77.8%) I, weak (55.6%) II. Kappa test k = 0,62 (p<0,05) interpreted as substantial agreement. Chi analysis there significant difference between IHC (p 0.002). Conclusion: decrease exposed for months